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Isolation and partial characterization of Borrelia burgdorferi inner and outer membranes by using isopycnic centrifugation.

In order to characterize the protein composition of the outer membrane of Borrelia burgdorferi, we have isolated inner and outer membranes by using discontinuous sucrose density step gradients. Outer and inner membrane fractions isolated by this method contained less than 1 and 2%, respectively, of...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Bledsoe, H A, Carroll, J A, Whelchel, T R, Farmer, M A, Dorward, D W, Gherardini, F C
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1994
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197199/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8002566/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.24.7447-7455.1994
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