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Cloning, nucleotide sequence, mutagenesis, and mapping of the Bacillus subtilis pbpD gene, which codes for penicillin-binding protein 4.

The gene encoding penicillin-binding protein 4 (PBP 4) of Bacillus subtilis, pbpD, was cloned by two independent methods. PBP 4 was purified, and the amino acid sequence of a cyanogen bromide digestion product was used to design an oligonucleotide probe for identification of the gene. An oligonucleo...

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Main Authors: Popham, D L, Setlow, P
格式: Artigo
語言:Inglês
出版: 1994
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC197107/
https://ncbi.nlm.nih.gov/pubmed/7961491
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