Single-step purification of Proteus mirabilis urease accessory protein UreE, a protein with a naturally occurring histidine tail, by nickel chelate affinity chromatography.

Proteus mirabilis urease, a nickel metalloenzyme, is essential for the virulence of this species in the urinary tract. Escherichia coli containing cloned structural genes ureA, ureB, and ureC and accessory genes ureD, ureE, ureF, and ureG displays urease activity when cultured in M9 minimal medium....

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Sriwanthana, B, Island, M D, Maneval, D, Mobley, H L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197051/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7961442/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.22.6836-6841.1994
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