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Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementin...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Tsinoremas, N F, Kutach, A K, Strayer, C A, Golden, S S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197036/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7961432/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.21.6764-6768.1994
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