Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism.

We constructed a series of transposon vectors which allow efficient in vitro gene manipulation and subsequent introduction of cloned DNA into a variety of gram-negative bacteria. Transfer of the cloned fragment from these multicopy plasmids into self-transmissible broad-host-range vectors is achieve...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Kok, M, Rekik, M, Witholt, B, Harayama, S
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1994
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197011/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7961407/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.21.6566-6571.1994
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