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Regulation of the Escherichia coli nrd operon: role of DNA supercoiling.

An in vitro RNA transcription assay was used to investigate the regulation of the expression of the nrd promoter. Using a linear DNA template, we found that Fis protein, which has a positive effect on expression of the nrd promoter in an nrd-lacZ fusion in vivo, had a moderate negative effect in vit...

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Autors principals: Sun, L, Fuchs, J A
Format: Artigo
Idioma:Inglês
Publicat: 1994
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC196282/
https://ncbi.nlm.nih.gov/pubmed/8045892
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