Molecular cloning and analysis of genes for sialic acid synthesis in Neisseria meningitidis group B and purification of the meningococcal CMP-NeuNAc synthetase enzyme.

The gene encoding for the CMP-NeuNAc synthetase enzyme of Neisseria meningitidis group B was cloned by complementation of a mutant of Escherichia coli defective for this enzyme. The gene (neuA) was isolated on a 4.1-kb fragment of meningococcal chromosomal DNA. Determination of the nucleotide sequen...

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Bibliographic Details
Main Authors: Ganguli, S, Zapata, G, Wallis, T, Reid, C, Boulnois, G, Vann, W F, Roberts, I S
Format: Artigo
Language:Inglês
Published: 1994
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC196278/
https://ncbi.nlm.nih.gov/pubmed/8045888
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