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Combinatorial mutagenesis of the lamB gene: residues 41 through 43, which are conserved in Escherichia coli outer membrane proteins, are informationally important in maltoporin structure and function.

A new strategy for combinatorial mutagenesis was developed and applied to residues 40 through 60 of LamB protein (maltoporin), with the aim of identifying amino acids important for LamB structure and function. The strategy involved a template containing a stop codon in the target sequence and a pool...

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Bibliographic Details
Main Authors: Chan, W C, Ferenci, T
Format: Artigo
Language:Inglês
Published: 1993
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC196231/
https://ncbi.nlm.nih.gov/pubmed/8423156
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