Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences.

A novel subtraction hybridization procedure, incorporating a combination of four separation strategies, was developed to isolate unique DNA sequences from a strain of Rhizobium leguminosarum bv. trifolii. Sau3A-digested DNA from this strain, i.e., the probe strain, was ligated to a linker and hybrid...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Bjourson, A J, Stone, C E, Cooper, J E
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1992
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195771/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1637166/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.7.2296-2301.1992
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