In vitro scanning saturation mutagenesis of an antibody binding pocket

We have combined PCR mutagenesis with in vitro transcription/translation and ELISA for the rapid generation and characterization of antibody mutants. The PCR products are used directly as the template for the in vitro transcription/translation reactions and because no cloning steps are required, the...

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Bibliografske podrobnosti
Main Authors: Burks, Elizabeth A., Chen, Gang, Georgiou, George, Iverson, Brent L.
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences of the USA 1997
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC19525/
https://ncbi.nlm.nih.gov/pubmed/9012796
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