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Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics

A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 Å resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong t...

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Detalhes bibliográficos
Main Authors: Nango, Eriko, Kumasaka, Takashi, Sato, Takao, Tanaka, Nobuo, Kakinuma, Katsumi, Eguchi, Tadashi
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952460/
https://ncbi.nlm.nih.gov/pubmed/16511136
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105018841
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