Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes

The tRNA-specific adenosine deaminase from the pathogenic bacteria Streptococcus pyogenes (spTAD) has been overexpressed in Escherichia coli and crystallized in the presence of Zn(2+) ion at 295 K using ammonium sulfate as a precipitant. Flash-cooled crystals of spTAD diffracted to 2.0 Å using 30%(v...

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Detalhes bibliográficos
Main Authors: Ku, Min-Je, Lee, Won-Ho, Nam, Ki-hyun, Rhee, Kyeong-hee, Lee, Ki-Seog, Kim, Eunice EunKyung, Yu, Myung-Hee, Hwang, Kwang Yeon
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952419/
https://ncbi.nlm.nih.gov/pubmed/16511045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105007311
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