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Crystallization and preliminary X-ray analysis of hyperthermophilic l-threonine dehydrogenase from the archaeon Pyrococcus horikoshii

Recombinant l-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii was prepared using an Escherichia coli expression system. The hyperthermostable l-threonine dehydrogenase consists of 348 amino acids with a molecular weight of 37.7 kDa. The enzyme was crystallized by th...

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Autores principales: Higashi, Noriko, Matsuura, Takanori, Nakagawa, Atsushi, Ishikawa, Kazuhiko
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2005
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952418/
https://ncbi.nlm.nih.gov/pubmed/16511061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910500881X
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