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Crystallization and preliminary X-ray characterization of d-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi

A recombinant form of d-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 Å....

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Detalhes bibliográficos
Main Authors: Nakajima, Yoshitaka, Ito, Kiyoshi, Ichihara, Emi, Ogawa, Kyohei, Egawa, Takashi, Xu, Yue, Yoshimoto, Tadashi
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952369/
https://ncbi.nlm.nih.gov/pubmed/16508084
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104024741
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