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Cloning, expression, purification and preliminary crystallographic data for Rv3214 (EntD), a predicted cofactor-dependent phosphoglycerate mutase from Mycobacterium tuberculosis

The Mycobacterium tuberculosis open reading frame Rv3214, annotated as a cofactor-dependent phosphoglycerate mutase, has been cloned and expressed as an N-terminally His-tagged protein. Tagged, untagged and selenomethionine-labelled forms of Rv3214 (EntD) have been purified using nickel-affinity chr...

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Detalhes bibliográficos
Main Authors: Watkins, Harriet A., Yu, MinMin, Baker, Edward N.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952354/
https://ncbi.nlm.nih.gov/pubmed/16511148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105020646
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