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Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-­finger zinc-finger peptide, bound to DNA

Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped...

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Main Authors: Crotty, Justin W., Etzkorn, Christopher, Barbas, Carlos F., Segal, David J., Horton, Nancy C.
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2005
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952332/
https://ncbi.nlm.nih.gov/pubmed/16511099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105014363
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