Isolation, crystallization and preliminary X-ray analysis of a methanol-induced corrinoid protein from Moorella thermoacetica

A corrinoid protein was induced and overexpressed in methanol-grown cells of the thermophilic anaerobic bacterium Moorella thermoacetica. The protein was purified from cytosolic extracts. After screening for crystallization conditions and optimization, crystals were obtained that diffracted strongly...

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Bibliographic Details
Main Authors: Zhou, Weihong, Das, Amaresh, Habel, Jeff E., Liu, Zhi-Jie, Chang, Jessie, Chen, Lirong, Lee, Doowon, Nguyen, Duong, Chang, Shu-Huey, Tempel, Wolfram, Rose, John P., Ljungdahl, Lars G., Wang, Bi-Cheng
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2005
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952305/
https://ncbi.nlm.nih.gov/pubmed/16511090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105010511
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