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Purification, crystallization and preliminary crystallographic analysis of DehIVa, a dehalogenase from Burkholderia cepacia MBA4

DehIVa is one of two dehalogenases produced by the soil- and water-borne bacterium Burkholderia cepacia MBA4. It acts to break down short-chain halogenated aliphatic acids through a nucleophilic attack and subsequent hydrolysis of an enzyme–substrate intermediate to remove the halide ions from l-ena...

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Autors principals: Schmidberger, Jason W., Oakley, Aaron J., Tsang, Jimmy S. H., Wilce, Matthew C. J.
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952274/
https://ncbi.nlm.nih.gov/pubmed/16511015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105002472
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