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Acetamide Selection of Kluyveromyces lactis Cells Transformed with an Integrative Vector Leads to High-Frequency Formation of Multicopy Strains

The yeast Kluyveromyces lactis has been extensively used as a host for heterologous protein expression. A necessary step in the construction of a stable expression strain is the introduction of an integrative expression vector into K. lactis cells, followed by selection of transformed strains using...

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Detalhes bibliográficos
Main Authors: Read, Jeremiah D., Colussi, Paul A., Ganatra, Mehul B., Taron, Christopher H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950971/
https://ncbi.nlm.nih.gov/pubmed/17586678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02253-06
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