Virus detection and identification using random multiplex (RT)-PCR with 3'-locked random primers

BACKGROUND: PCR-based detection and identification of viruses assumes a known, relatively stable genome. Unfortunately, high mutation rates may lead to extensive changes in viral nucleic acid sequences making dedicated PCR primer use problematic. Furthermore, in bioterrorism, viral consensus sequenc...

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Bibliografiska uppgifter
Huvudupphovsmän: Clem, Amy L, Sims, Jonathan, Telang, Sucheta, Eaton, John W, Chesney, Jason
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2007
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950496/
https://ncbi.nlm.nih.gov/pubmed/17598900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-4-65
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