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Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens

BACKGROUND: Over the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead...

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Autors principals: Evans, Mark F, Aliesky, Holly A, Cooper, Kumarasen
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC194893/
https://ncbi.nlm.nih.gov/pubmed/12801424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6890-3-2
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