Detection of Mycoplasma pneumoniae by Real-Time Nucleic Acid Sequence-Based Amplification

Real-time isothermal nucleic acid sequence-based amplification (RT-NASBA) was applied to the detection of Mycoplasma pneumoniae. In vitro-generated M. pneumoniae RNA was used to assess the sensitivity of the assay. The 95% hit rate was 148 molecules of M. pneumoniae RNA in the amplification and 10(4...

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Publicado en:	J Clin Microbiol
Autores principales:	Loens, K., Ieven, M., Ursi, D., Beck, T., Overdijk, M., Sillekens, P., Goossens, H.
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2003
Materias:	Bacteriology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193797/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12958290/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.9.4448-4450.2003
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