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Application of RNase in the purification of RNA-binding proteins

BASIC FINDINGS: It was found that RNA-binding proteins can be contaminated with host RNA during purification. The contamination of purified RNA-binding protein with RNA was identified by gel electrophoresis and EtBr staining. Our data suggest that applications of appropriate enzymes (DNase or RNase)...

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Detalhes bibliográficos
Main Authors: Kang, Jonghoon, Lee, Myung Soog, Gorenstein, David G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1933585/
https://ncbi.nlm.nih.gov/pubmed/17400170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.03.003
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