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NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants

The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers...

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Bibliographic Details
Main Authors: Chen, Yung-Fu, Chen, Rung-Ching, Tseng, Lin-Yu, Lin, Elong, Chan, Yung-Kuan, Pan, Ren-Hao
Format: Artigo
Language:Inglês
Published: Oxford University Press 2007
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1933230/
https://ncbi.nlm.nih.gov/pubmed/17488836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm305
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