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Sonoporation Is an Efficient Tool for Intracellular Fluorescent Dextran Delivery and One-Step Double-Crossover Mutant Construction in Fusobacterium nucleatum

Studies of microorganisms are often hindered by a lack of effective genetic tools. One such example is Fusobacterium nucleatum, a gram-negative anaerobe associated with various human infections, including those causing periodontal disease and preterm birth. The first double-crossover allelic-exchang...

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Detalhes bibliográficos
Main Authors: Han, Yiping W., Ikegami, Akihiko, Chung, Peter, Zhang, Lei, Deng, Cheri X.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1932673/
https://ncbi.nlm.nih.gov/pubmed/17449701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00428-07
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