A method for assaying intestinal brush-border sucrase in an intact intestinal preparation

Small intestinal brush-border hydrolases usually are assayed in intestinal mucosal homogenates resuspended in solutions of unphysiological ionic composition. Thus, extrapolation of measured V(max) values (maximal reaction rates at high substrate concentrations) to in vivo conditions, hence compariso...

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Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Lee, Eric A., Weiss, Stacey L., Lam, Mandy, Torres, Rosa, Diamond, Jared
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 1998
Предметы:	Biological Sciences
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19267/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9482847/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.5.2111
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