Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth

We have developed a microfluidic device that allows the isolation and genome amplification of individual microbial cells, thereby enabling organism-level genomic analysis of complex microbial ecosystems without the need for culture. This device was used to perform a directed survey of the human subg...

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Dettagli Bibliografici
Autori principali: Marcy, Yann, Ouverney, Cleber, Bik, Elisabeth M., Lösekann, Tina, Ivanova, Natalia, Martin, Hector Garcia, Szeto, Ernest, Platt, Darren, Hugenholtz, Philip, Relman, David A., Quake, Stephen R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2007
Soggetti:
Physical Sciences
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1924555/
https://ncbi.nlm.nih.gov/pubmed/17620602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704662104
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