Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast.

The dengue virus type 2 genomic RNA was amplified by reverse transcription-PCR and cloned as four cDNA fragments. We could not assemble these four fragments into full-length cDNA in Escherichia coli. The full-length dengue virus cDNA was constructed by homologous recombination in yeast, either as pa...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Polo, S, Ketner, G, Levis, R, Falgout, B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC191775/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9188607/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.71.7.5366-5374.1997
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