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Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: Comparison with denaturing gradient gel electrophoresis and nucleotide sequencing

Previously, an assay called conformation sensitive gel electrophoresis (CSGE) was developed for scanning PCR products for the presence of single-base and larger base mismatches in DNA. The assay was based on the assumption that mildly denaturing solvents in an appropriate buffer can accentuate the c...

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Main Authors: Körkkö, Jarmo, Annunen, Susanna, Pihlajamaa, Tero, Prockop, Darwin J., Ala-Kokko, Leena
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 1998
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC19147/
https://ncbi.nlm.nih.gov/pubmed/9465076
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