Systematic Colocalization Errors between Acridine Orange and EGFP in Astrocyte Vesicular Organelles

Dual-color imaging of acridine orange (AO) and EGFP fused to a vesicular glutamate transporter or the vesicle-associated membrane proteins 2 or 3 has been used to visualize a supposedly well-defined subpopulation of glutamatergic astrocytic secretory vesicles undergoing regulated exocytosis. However...

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Detalhes bibliográficos
Main Authors: Nadrigny, Fabien, Li, Dongdong, Kemnitz, Klaus, Ropert, Nicole, Koulakoff, Annette, Rudolph, Stephanie, Vitali, Marco, Giaume, Christian, Kirchhoff, Frank, Oheim, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2007
Assuntos:
Spectroscopy, Imaging, Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1913145/
https://ncbi.nlm.nih.gov/pubmed/17416619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.102673
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