Towards Quantitative mRNA Analysis in Paraffin-Embedded Tissues Using Real-Time Reverse Transcriptase-Polymerase Chain Reaction : A Methodological Study on Lymph Nodes from Melanoma Patients

Improved extraction techniques combined with sensitive real-time reverse transcriptase-polymerase chain reaction may allow detection of mRNA in formalin-fixed, paraffin-embedded (FFPE) materials, but the factors affecting mRNA quantification in clinical material using these methods have not been sys...

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Bibliographic Details
Main Authors: Abrahamsen, Helene Nortvig, Steiniche, Torben, Nexo, Ebba, Hamilton-Dutoit, Stephen J., Sorensen, Boe Sandahl
Format: Artigo
Language:Inglês
Published: American Society for Investigative Pathology 2003
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Regular Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1907376/
https://ncbi.nlm.nih.gov/pubmed/12552078
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