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High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni

A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determina...

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Detalhes bibliográficos
Main Authors: Price, Erin P., Smith, Helen, Huygens, Flavia, Giffard, Philip M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1907115/
https://ncbi.nlm.nih.gov/pubmed/17400785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02702-06
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