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Complete replication in vitro of tobacco mosaic virus RNA by a template-dependent, membrane-bound RNA polymerase.

A crude membrane-bound RNA polymerase, obtained by differential centrifugation of extracts of tomato leaves infected with tobacco mosaic tobamovirus (tomato strain L) TMV-L), was purified by sucrose density gradient centrifugation. Removal of the endogenous RNA template with micrococcal nuclease ren...

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Detalhes bibliográficos
Publicado no:J Virol
Principais autores: Osman, T A, Buck, K W
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190647/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8709249/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.9.6227-6234.1996
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