Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

BACKGROUND: Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dilution. RESULTS: Hansenula polymorpha (Pichia angusta) muta...

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Main Authors: Dmytruk, Kostyantyn V, Smutok, Oleh V, Ryabova, Olena B, Gayda, Galyna Z, Sibirny, Volodymyr A, Schuhmann, Wolfgang, Gonchar, Mykhailo V, Sibirny, Andriy A
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2007
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1905912/
https://ncbi.nlm.nih.gov/pubmed/17567895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-33
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