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Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.

A genomic clone of the small, round-structured virus Southampton virus (SV) was constructed from a set of overlapping PCR amplicons. Sequence analysis confirmed the absence of mutations and accurate ligation of the PCR products. The SV cDNA was cloned into a vector for in vitro production of RNA and...

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Detalhes bibliográficos
Publicado no:J Virol
Principais autores: Liu, B, Clarke, I N, Lambden, P R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190109/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8642693/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.4.2605-2610.1996
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