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Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
Inactivation of gene products by dominant-negative (DN) mutants is a powerful tool to assign functions to proteins. Here, we present a two-step procedure to establish a random screen for DN alleles, using the essential murine cytomegalovirus gene M50 as an example. First, loss-of-function mutants fr...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2007
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1900260/ https://ncbi.nlm.nih.gov/pubmed/17376929 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.02796-06 |
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