Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50

Inactivation of gene products by dominant-negative (DN) mutants is a powerful tool to assign functions to proteins. Here, we present a two-step procedure to establish a random screen for DN alleles, using the essential murine cytomegalovirus gene M50 as an example. First, loss-of-function mutants fr...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Rupp, Brigitte, Ruzsics, Zsolt, Buser, Christopher, Adler, Barbara, Walther, Paul, Koszinowski, Ulrich H.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2007
Pynciau:
Structure and Assembly
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1900260/
https://ncbi.nlm.nih.gov/pubmed/17376929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.02796-06
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