Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.

A specific and rapid method for detecting the presence of cytomegalovirus (CMV) DNA in formalin- or mercuric chloride-fixed, paraffin-embedded tissue has been developed. With dot hybridization with a biotinylated CMV DNA probe, the presence of CMV infection can be readily detected even if only a min...

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Main Authors: Seto, E., Yen, T. S.
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899767/
https://ncbi.nlm.nih.gov/pubmed/3035929
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spelling pubmed-18997672007-06-27 Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization. Seto, E. Yen, T. S. Am J Pathol Research Article A specific and rapid method for detecting the presence of cytomegalovirus (CMV) DNA in formalin- or mercuric chloride-fixed, paraffin-embedded tissue has been developed. With dot hybridization with a biotinylated CMV DNA probe, the presence of CMV infection can be readily detected even if only a minority of the cells in the tissue block are infected. The sensitivity is further increased by about two orders of magnitude if high specific-activity 32P-labeled probe is used. This method of detection is more sensitive than histologic diagnosis, appears to be roughly comparable to that by virus isolation in culture, and should be applicable to the detection of other infectious agents. 1987-06 /pmc/articles/PMC1899767/ /pubmed/3035929 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
Seto, E.
Yen, T. S.
Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
description A specific and rapid method for detecting the presence of cytomegalovirus (CMV) DNA in formalin- or mercuric chloride-fixed, paraffin-embedded tissue has been developed. With dot hybridization with a biotinylated CMV DNA probe, the presence of CMV infection can be readily detected even if only a minority of the cells in the tissue block are infected. The sensitivity is further increased by about two orders of magnitude if high specific-activity 32P-labeled probe is used. This method of detection is more sensitive than histologic diagnosis, appears to be roughly comparable to that by virus isolation in culture, and should be applicable to the detection of other infectious agents.
author Seto, E.
Yen, T. S.
author_facet Seto, E.
Yen, T. S.
author_sort Seto, E.
title Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
title_short Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
title_full Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
title_fullStr Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
title_full_unstemmed Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.
title_sort detection of cytomegalovirus infection by means of dna isolated from paraffin-embedded tissues and dot hybridization.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1899767/
https://ncbi.nlm.nih.gov/pubmed/3035929
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