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A flow cytometry-based method for detecting antibody responses to murine cytomegalovirus infection

An assay based on target cells infected with green fluorescent protein labeled murine cytomegalovirus (GFP-MCMV) and dual color flow cytometry for detecting antibody to MCMV is described. After optimizing conditions for this technique, kinetics of anti-MCMV IgG antibody response were tested in susce...

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Bibliographic Details
Main Authors: Bickerstaff, Alice A., Zimmerman, Peter D., Wing, Bret A., Taylor, Frederick, Trgovcich, Joanne, Cook, Charles H.
Format: Artigo
Language:Inglês
Published: 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899410/
https://ncbi.nlm.nih.gov/pubmed/17303259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.01.006
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