Improved Luciferase Tagging System for Listeria monocytogenes Allows Real-Time Monitoring In Vivo and In Vitro

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during...

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Detalhes bibliográficos
Main Authors: Riedel, Christian U., Monk, Ian R., Casey, Pat G., Morrissey, David, O'Sullivan, Gerald C., Tangney, Mark, Hill, Colin, Gahan, Cormac G. M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Food Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892880/
https://ncbi.nlm.nih.gov/pubmed/17351089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02940-06
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