Base-specific spin-labeling of RNA for structure determination

To facilitate the measurement of intramolecular distances in solvated RNA systems, a combination of spin-labeling, electron paramagnetic resonance (EPR), and molecular dynamics (MD) simulation is presented. The fairly rigid spin label 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was base...

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Autors principals: Piton, Nelly, Mu, Yuguang, Stock, Gerhard, Prisner, Thomas F., Schiemann, Olav, Engels, Joachim W.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2007
Matèries:
RNA
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1891445/
https://ncbi.nlm.nih.gov/pubmed/17452362
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm169
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