An improved technique for the in situ detection of DNA after polymerase chain reaction amplification.

In situ detection of polymerase chain reaction (PCR)-amplified DNA in cell and tissue preparations previously required 5 to 7 primer pairs designed to generate a long (greater than 1,000 base pair) product. The authors describe a nonisotopic PCR in situ technique, employing a single primer pair and...

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Detalles Bibliográficos
Autores principales: Nuovo, G. J., Gallery, F., MacConnell, P., Becker, J., Bloch, W.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1991
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1886461/
https://ncbi.nlm.nih.gov/pubmed/1661072
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