Use of biotinylated beta-lactams and chemiluminescence for study and purification of penicillin-binding proteins in bacteria.
A new reagent for the detection of penicillin-binding proteins (PBPs) was developed. An N-hydroxysuccinimide ester of biotin was used to tag beta-lactam antibiotics with free side chain amino groups such as ampicillin (BIO-AMP), 6-aminopenicillanic acid (BIO-APA), and 7-aminocephalosporanic acid (BI...
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| Publicado no: | Antimicrob Agents Chemother |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC188136/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8067779/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.38.5.973 |
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