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Quantitative Proteome Analysis Using d-Labeled N-Ethylmaleimide and (13)C-Labeled Iodoacetanilide by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

A new methodology for quantitative analysis of proteins is described, applying stable isotope labeling by small organic molecules combined with 1- or 2-dimensional electrophoresis and MALDI-TOF-MS, also allowing concurrent protein identification by peptide mass fingerprinting. Our method eliminates...

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Detalhes bibliográficos
Main Authors: Kurono, Sadamu, Kurono, Tamie, Komori, Naoka, Niwayama, Satomi, Matsumoto, Hiroyuki
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1876768/
https://ncbi.nlm.nih.gov/pubmed/17049249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2006.09.023
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