Cloning of a Streptomyces clavuligerus DNA fragment encoding the cephalosporin 7 alpha-hydroxylase and its expression in Streptomyces lividans.

A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this organism. The library was constructed in the lambda GEM-11 phage system. After plaque purification and reprob...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Xiao, X, Hintermann, G, Häusler, A, Barker, P J, Foor, F, Demain, A L, Piret, J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC187609/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8431021/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.37.1.84
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