Cloning of a Streptomyces clavuligerus DNA fragment encoding the cephalosporin 7 alpha-hydroxylase and its expression in Streptomyces lividans.

A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this organism. The library was constructed in the lambda GEM-11 phage system. After plaque purification and reprob...

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Bibliographic Details
Main Authors: Xiao, X, Hintermann, G, Häusler, A, Barker, P J, Foor, F, Demain, A L, Piret, J
Format: Artigo
Language:Inglês
Published: 1993
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC187609/
https://ncbi.nlm.nih.gov/pubmed/8431021
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