Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kγ Origin of Replication

Bacterial artificial chromosome (BAC) mediated transgenesis has proven to be a highly reliable way to obtain accurate transgene expression for in vivo studies of gene expression and function. A rate-limiting step in use of this technology to characterize large numbers of genes has been the process w...

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Bibliographic Details
Main Authors: Gong, Shiaoching, Yang, Xiangdong William, Li, Chenjian, Heintz, Nathaniel
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2002
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC187570/
https://ncbi.nlm.nih.gov/pubmed/12466304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.476202
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