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Mg(2+)-dependent folding of a Diels-Alderase ribozyme probed by single-molecule FRET analysis
Here, we report a single-molecule fluorescence resonance energy transfer (FRET) study of a Diels-Alderase (DAse) ribozyme, a 49-mer RNA with true catalytic properties. The DAse ribozyme was labeled with Cy3 and Cy5 as a FRET pair of dyes to observe intramolecular folding, which is a prerequisite for...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1874616/ https://ncbi.nlm.nih.gov/pubmed/17344321 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm072 |
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