Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes

To investigate the feasibility of conducting a genomic-scale protein labeling and localization study in Escherichia coli, a representative subset of 23 coding DNA sequences (CDSs) was selected for chromosomal tagging with one or more fluorescent protein genes (EGFP, EYFP, mRFP1, DsRed2). We used λ-R...

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Autores principales: Watt, Rory M., Wang, Jing, Leong, Meikid, Kung, Hsiang-fu, Cheah, Kathryn S.E., Liu, Depei, Danchin, Antoine, Huang, Jian-Dong
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1874593/
https://ncbi.nlm.nih.gov/pubmed/17272300
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl1158
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