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Two-Dimensional Polyacrylamide Gel Electrophoresis of Envelope Proteins of Escherichia coli

A method of separating envelope proteins by two-dimensional polyacrylamide gel electrophoresis is described. Escherichia coli envelopes (inner and outer membranes) were prepared by French pressing and washed by repeated centrifugation. Membrane proteins were solubilized with guanidine thiocyanate an...

詳細記述

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書誌詳細
出版年:Appl Microbiol
主要な著者: Johnson, W. Charles, Silhavy, T. J., Boos, W.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1975
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC186988/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/803821/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.29.3.405-413.1975
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