Establishment of Real-Time Polymerase Chain Reaction Method for Quantitative Analysis of Asparagine Synthetase Expression

We established a real-time quantitative PCR (RQ-PCR) with which to measure abundance of the asparagine synthetase (AS) mRNA. The level of AS mRNA paralleled AS enzyme activity, as well as the AS protein level detected by Western blotting and by in situ immunostaining. Cytotoxicity tests in vitro sho...

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Detaylı Bibliyografya
Asıl Yazarlar: Irino, Tamotsu, Kitoh, Toshiyuki, Koami, Kenichi, Kashima, Terumi, Mukai, Kouichi, Takeuchi, Eiji, Hongo, Teruaki, Nakahata, Tatsutoshi, Schuster, Sheldon M., Osaka, Mitsuhiko
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Investigative Pathology 2004
Konular:
Regular Articles
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1867639/
https://ncbi.nlm.nih.gov/pubmed/15269298
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