Establishment of Real-Time Polymerase Chain Reaction Method for Quantitative Analysis of Asparagine Synthetase Expression

We established a real-time quantitative PCR (RQ-PCR) with which to measure abundance of the asparagine synthetase (AS) mRNA. The level of AS mRNA paralleled AS enzyme activity, as well as the AS protein level detected by Western blotting and by in situ immunostaining. Cytotoxicity tests in vitro sho...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Irino, Tamotsu, Kitoh, Toshiyuki, Koami, Kenichi, Kashima, Terumi, Mukai, Kouichi, Takeuchi, Eiji, Hongo, Teruaki, Nakahata, Tatsutoshi, Schuster, Sheldon M., Osaka, Mitsuhiko
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: American Society for Investigative Pathology 2004
विषय:
Regular Articles
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC1867639/
https://ncbi.nlm.nih.gov/pubmed/15269298
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